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(A) Flowchart of candidate miRNA selection. <t>Microarray</t> results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.
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(A) Flowchart of candidate miRNA selection. <t>Microarray</t> results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.
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a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The <t>microRNA</t> profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
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(A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

Journal: PLOS ONE

Article Title: MicroRNA-26a-5p is a reliable biomarker in the adjuvant setting for pancreatic ductal adenocarcinoma

doi: 10.1371/journal.pone.0310328

Figure Lengend Snippet: (A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

Article Snippet: To investigate candidate miRNAs, microarray analyses of miRNA expression in 18 blood samples ( ) was performed using the Toray microRNA microarray system (Toray Industries, Tokyo, Japan).

Techniques: Selection, Microarray, Adjuvant, Isolation

a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

Journal: Cell Death & Disease

Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

doi: 10.1038/s41419-024-06744-0

Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition